Well, today was rough. Tried to get up to go into work early, set the alarm for 7:15am, but just couldn’t get up… I was exhausted. Last night I spent 3.5 hours doing laundry and hanging it up and folding…. ugh! Six loads of laundry in the washer and 4 dryer loads! But now everything we own is clean and the towels are fluffy…. and I’ve finished the sixth book of the Wheel of Time :D
It wouldn’t have mattered had I gone in early anyway because I didn’t want to risk sing my EDTA to do sequencing again because my sequencing didn’t work last time, so I was going to borrow Weihui’s, but he doesn’t get in until 9am. I finished up the sequencing around 10:45am and head over to NAPS to run. I should have the results tomorrow… this is my last opportunity to try to get the promoter region sequence in time to submit an abstract to the SFN conference! Hopefully it works, I was very careful! In the Monday afternoon lab meeting I was told some ideas for primers, I designed them on Monday, they were done on Wednesday afternoon and I ran the sequencing PCR overnight, and then today I finished it up *crosses fingers*
It was a long day though… I didn’t sign up fast enough for the PCR machine, so I had to run my samples from 1:30 to 4pm, then start the gel extraction, which took me until 7pm. I’ve got the digest started, so tomorrow I can purify it and ligate, on Saturday I can transform the E. coli, screen the clones on Sunday, and on Monday I can have the miniprep done VERY EARLY, like be in the lab by 6am, heh… and I’ll have the data in time. What a rush!
