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Well, this was my first full week in the Song lab and it was ok. I had my midterm on Monday which was a big stress relief! I ran several PCR gels… hmmm… I have 4 primers and ran test runs on those, 2 worked so I went ahead and amplified a lot of that DNA, but only one of them worked! I didn’t know you had to use the same PCR machine (there are two in the lab) but even trying the one that didn’t work with the EXACT SAME SETTINGS on the original PCR machine still nothing amplified… weird. The two which didn’t work originally I ran another test PCR and got one of them working, so I’m leaving the two which don’t work for awhile and will go back to them later.
For the original one which worked I amplified a bunch and even isolated the DNA! Cool stuff. I just kind of followed a recipe and asked everyone questions along the way because Fang wasn’t in on Friday. I was going to clone the fragment into the vector then, but we were out of the restriction enzyme I needed and the place where you buy it was closed. Weihui showed me where all the cloning stuff was and how to pick a buffer, and he was extremely patient and a good teacher (fang can sometimes go too quickly on things, heh) I told him I’ll need his help next week with cloning and he said he would.
You can see a picture of the gel from my first PCR reaction on the left! Cool huh?!
